Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 May 31;12:83. doi: 10.1186/s40478-024-01784-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — Preparation of HPMB-OSCs. (a) A tissue block of the middle frontal gyrus is dissected during autopsy and cut into a block of roughly 1.5 × 1.5 cm. (b) A vibratome is used to cut 300 μm-thick slices. (c) Slices are transferred onto semi-permeable membrane inserts in 6-well culture plates containing slice culture medium. (d) hCSF is collected at autopsy from the lateral ventricles using a syringe and spinal needle and slices are cultured with or without hCSF supplementation to the culture medium. (e) Timeline of the experimental procedure: slices are prepared and cultured up to 42 DIV. Slices are fixed in PFA once every week and slice-conditioned medium is collected for further analysis