FIG. 2.
Poly P accumulations in V. cholerae. (A) Following overnight culture in LB, the wild-type strain 92A1552-Rifr was subcultured into fresh LB at a 1:100 dilution and grown at 37°C with aeration. (B and C) Following overnight culture in MOPS medium with 0.1 mM Pi, the wild-type strain was subcultured in MOPS medium with 0.1 (squares), 0.5 (circles), or 2 (triangles) mM Pi. Solid symbols, growth (A540); open symbols, poly P. (D) Following overnight culture in MOPS with 0.1 mM Pi, the wild-type (squares) and KVC3 (ppk mutant [circles]) strains were harvested and washed with MOPS buffer. The cells were transferred to Pi-free MOPS and cultivated for 1 h at 37°C with aeration. Potassium phosphate buffer (pH 7.4) was then added to a final concentration of 20 mM Pi at zero hour. Solid symbols, growth (A600); open symbols, poly P.