FIG. 4.

V. cholerae ppk complements the adaptive growth defect in an E. coli ppk mutant. E. coli strains MG1655(pFYZ1) (squares), CF5802(pFYZ1) (open circles), and CF5802(pVCK37) (solid circles) were grown in 2×YT medium supplemented with 50 μg of ampicillin/ml to an A₆₀₀ of 0.5. The cells were harvested and washed twice with MOPS medium devoid of nutrients and resuspended in MOPS medium with 2 mM P_i and 0.4% glucose at zero hour.