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Growth adaptation to an excess amount of calcium. V. cholerae strains 92A1552-Rifr(pBluescript II) (squares), KVC3(pBluescript II) (open circles), and KVC3(pVCK31) (solid circles) were grown in LB medium supplemented with 10 mM KH2PO4 and 50 μg of ampicillin/ml overnight. The cells were inoculated in LB supplemented with 200 mM CaCl2 and shaken at 37°C.
