TABLE 1.

PPK and PPX activities and poly P levels in V. cholerae and E. coli^a

Strain	Genotypeb	PPK activity (U/mg)		PPX activity (U/mg)	Poly P level (nmol/mg)
		Soluble	Membrane
V. cholerae
92A1552-Rifr	WT	1,120	17,300	<30	25.3
KVC3	ppk::Kan	<30	<300	<30	<0.10
E. coli MG1655	WT	550	14,000	1,200

^aPPK and PPX activities were determined using cell extracts prepared from stationary-phase cultures in LB, and poly P levels were determined using cell extracts prepared from stationary-phase cultures in 2 mM P_i MOPS medium. 

^bWT, wild type.