TABLE 1.
Strain | Genotypeb | PPK activity (U/mg)
|
PPX activity (U/mg) | Poly P level (nmol/mg) | |
---|---|---|---|---|---|
Soluble | Membrane | ||||
V. cholerae | |||||
92A1552-Rifr | WT | 1,120 | 17,300 | <30 | 25.3 |
KVC3 | ppk::Kan | <30 | <300 | <30 | <0.10 |
E. coli MG1655 | WT | 550 | 14,000 | 1,200 |
PPK and PPX activities were determined using cell extracts prepared from stationary-phase cultures in LB, and poly P levels were determined using cell extracts prepared from stationary-phase cultures in 2 mM Pi MOPS medium.
WT, wild type.