Figure 4.

Protein identification using western blotting and lysosomal enzymatic Assays

(A) Western blots were performed on N = 3 biological samples of each sham and AF group. Rab11A (24–25 kDa) and GAPDH control (37 kDa) were detected.

(B) The normalized band intensities of Rab11A data are presented as mean ± SD. The normalized band intensity value for the control group was 0.61 ± 0.20, and for the AF group at 0.90 ± 0.20. After performing a one-way t-test, the normalized protein band intensity showed a trend toward a significant upregulation in the AF group (p = 0.07).

(C) β galactosidase activity is displayed as mean ± SD. The AF group showed an enzymatic activity of 2.64, 3.02 and 3.26 nmol/min/mg, respectively, and the sham group showed 2.48, 2.57 and 5.68 nmol/min/mg. The mean enzymatic activity was 2.57 ± 0.31 for the AF group and 3.57 ± 1.8 for the control group. After performing a Two-way t-test, the β galactosidase activity of the AF group showed no significant regulation (p = 0.60).

(D) β hexosaminidase type-A activity of the AF group (N = 3) showed 6.66, 6.74 and 6.87 nmol/min/mg, respectively, and the sham group showed 5.4, 10.61 and 10.78 nmol/min/mg activity. The normalized mean enzymatic activity was at 8.93 ± 3.1 for the control group and 6.75 ± 0.11 for the AF group, and β hexosaminidase type- A activity of the AF group showed no significant regulation (p = 0.28).

(E) AF group showed (N = 3) 1.52, 1.66 and 1.73 nmol/min/mg of β hexosaminidase type B activity, and the sham group (N = 3) showed 1.5, 1.62 and 2.23 nmol/min/mg of β hexosaminidase type B activity, The mean enzymatic activity was at 1.8 ± 0.39 for the control group and 1.6 ± 0.11 for AF group. No significant regulation was observed in β hexosaminidase type B activity (p = 0.56).