Table 1.

Summary of GC-TOF-MS method validation.

Method Validation	Parameters	Fecal sample conditions	Plasma sample conditions
Matrix effect (%)a	The gradient of calibration curve from extracted matrix solvent compared with DE solvent	429 − 600	118 − 143
Recovery (%)a	Spiked mix standards before extraction	73 − 88	93 − 134
Sensitivityb	LOD	5 − 50 ng/mL	0.007 − 5.14 µM
	LOQ	14 − 152 ng/mL	0.024 − 17.133 µM
Precision (%RSD)b	Peak Area, Intraday (N = 10)	1 − 3	0 − 6
	Peak Area, Interday (N = 3)	6 − 11	2 − 17
	RT, Intraday (N = 10)	0.00 − 0.28	0.00 − 0.29
	RT, Interday (N = 3)	0.00 − 0.01	0.00 − 0.47

DE, diethyl ether; LOD, limit of detection; LOQ, limit of quantification; RSD, relative standard deviation; RT, retention time;

^aRefers to full detail in Table S3

^bRefers to full detail in Table S4