Table 1.
Study cohort of 29 laboratory workers immunized with first- or third-generation smallpox vaccines. Geometric mean titers (GMT) of reciprocal serum dilution of VACV- and MPXV nAb were calculated from two independent experiments with two replicates per dilution. Values displayed showed a viral neutralization >50 % compared to control. GMT values below 10 indicate that serum neutralization antibodies are below the limit of detection (1:10). Dash (−): no sample collected. Additional information on vaccination time, serum collections, and time between last vaccination and first serum collection are displayed in Supplementary Table S1.
| Participant | 1st or 3rd generation vaccine? | GMT VACV nAb Titer 2014 |
GMT VACV nAb titer 2015 |
GMT VACV nAb titer 2016 |
GMT VACV nAb titer 2017 |
GMT VACV nAb titer 2018 |
GMT VACV nAb titer 2019 |
GMT VACV nAb titer 2020 |
GMT VACV nAb titer 2021 |
GMT VACV nAb titer 2022 |
|---|---|---|---|---|---|---|---|---|---|---|
| GMT MPXV nAb titer 2014 | GMT MPXV nAb titer 2015 | GMT MPXV nAb titer 2016 | GMT MPXV nAb titer 2017 | GMT MPXV nAb titer 2018 | GMT MPXV nAb titer 2019 | GMT MPXV nAb titer 2020 | GMT MPXV nAb titer 2021 | GMT MPXV nAb titer 2022 | ||
| Participant 1 | First | – | 40 | 56.57 | 56.57 | – | 40 | – | 28.28 | – |
| – | 14.14 | 28.28 | 40 | – | 10 | – | 14.14 | – | ||
| Participant 2 | Third | – | 14.14 | 20 | 20 | 10 | 14.14 | 14.14 | 14.14 | 28.28 |
| – | 10 | 10 | 10 | 14.14 | 10 | 10 | 14.14 | 7.07 | ||
| Participant 3 | Third | – | – | – | – | – | – | – | – | 28.28 |
| – | – | – | – | – | – | – | – | 7.07 | ||
| Participant 4 | Third | – | – | 28.28 | 14.14 | 20 | 56.56 | 28.28 | 10 | 10 |
| – | – | 20 | 5 | 7.07 | 10 | 14.14 | 10 | 7.07 | ||
| Participant 5 | First | – | 40 | 56.56 | – | 80 | 56.56 | 28.28 | 20 | 56.56 |
| – | 20 | 80 | – | 20 | 20 | 40 | 28.28 | 28.28 | ||
| Participant 6 | First | – | – | – | 10 | 5 | – | – | – | – |
| – | – | – | 20 | 10 | – | – | – | – | ||
| Participant 7 | Third | – | – | – | – | – | 5 | 10 | 14.14 | 14.14 |
| – | – | – | – | – | 10 | 10 | 28.28 | 5 | ||
| Participant 8 | Third | – | – | – | – | – | – | – | – | 14.14 |
| – | – | – | – | – | – | – | – | 20 | ||
| Participant 9 | First | – | – | – | – | – | – | – | 28.28 | – |
| – | – | – | – | – | – | – | 20 | – | ||
| Participant 10 | Third | – | – | – | – | – | 14.14 | – | 14.14 | 28.28 |
| – | – | – | – | – | 5 | – | 10 | 14.14 | ||
| Participant 11 | Third | – | – | 80 | 10 | 14.14 | – | 28.28 | – | |
| – | – | 14.14 | 28.28 | 14.14 | – | 14.14 | – | – | ||
| Participant 12 | Third | – | – | 40 | 7.07 | 7.07 | 10 | 7.07 | – | 7.07 |
| – | – | 10 | 10 | 7.07 | 10 | 7.07 | – | 14.14 | ||
| Participant 13 | Third | – | – | 7.07 | 7.07 | 7.07 | 7.07 | 5 | 5 | 5 |
| – | – | 10 | 10 | 7.07 | 10 | 7.07 | 20 | 14.14 | ||
| Participant 14 | Third | 14.14 | – | – | – | – | – | – | – | – |
| 10 | – | – | – | – | – | – | – | – | ||
| Participant 15 | First | – | 20 | 80 | 56.57 | 20 | - | 14.14 | 14.14 | 80 |
| – | 28.28 | 56.57 | 56.57 | 28.28 | - | 28.28 | 28.28 | 80 | ||
| Participant 16 | Third | – | 7.07 | 7.07 | – | – | – | – | – | – |
| – | 14.14 | 14.14 | – | – | – | – | – | – | ||
| Participant 17 | Third | – | – | – | – | 10 | 7.07 | 20 | 14.14 | 7.07 |
| – | – | – | – | 7.07 | 5 | 7.07 | 7.07 | 10 | ||
| Participant 18 | First | – | 14.14 | 10 | 7.07 | 5 | 10 | 20 | – | – |
| – | 28.28 | 20 | 14.14 | 40 | 10 | 20 | – | – | ||
| Participant 19 | Third | – | – | – | – | – | – | 20 | 20 | 20 |
| – | – | – | – | – | – | 10 | 14.14 | 7.07 | ||
| Participant 20 | Third | – | – | 226.27 | 40 | 56.57 | 56.57 | 20 | 40 | 28.28 |
| – | – | 28.28 | 40 | 28.28 | 20 | 56.57 | 28.28 | 28.28 | ||
| Participant 21 | First | – | 56.57 | 28.28 | 56.57 | 40 | 160 | 160 | – | – |
| - | 40 | 28.28 | 40 | 28.28 | 56.57 | 80 | – | – | ||
| Participant 22 | First | – | 113.14 | 40 | 320 | 28.28 | 20 | – | – | – |
| – | 56.57 | 80 | 56.57 | 40 | 40 | – | – | – | ||
| Participant 23 | First | – | 56.57 | 160 | 56.57 | 56.57 | 28.28 | – | 40 | – |
| – | 40 | 40 | 28.28 | 40 | 20 | – | 28.28 | – | ||
| Participant 24 | Third | – | – | – | 5 | 5 | – | – | – | – |
| – | – | – | 10 | 40 | – | – | – | – | ||
| Participant 25 | Third | – | – | – | – | 7.07 | – | – | 7.07 | – |
| – | – | – | – | 5 | – | – | 10 | – | ||
| Participant 26 | Third | – | 5 | 5 | 14.14 | 7.07 | 7.07 | – | 14.14 | 10 |
| – | 7.07 | 14.14 | 10 | 20 | 14.14 | – | 20 | 14.14 | ||
| Participant 27 | Third | – | – | 10 | 5 | 10 | 10 | 10 | 20 | 20 |
| – | – | 10 | 14.14 | 7.07 | 10 | 10 | 14.14 | 10 | ||
| Participant 28 | First | – | – | 40 | 20 | 56.57 | 40 | 28.28 | 40 | 40 |
| – | – | 20 | 20 | 28.28 | 40 | 28.28 | 28.28 | 56.57 | ||
| Participant 29 | Third | – | – | – | – | – | – | 20 | 7.07 | 14.14 |
| – | – | – | – | – | – | 14.14 | 40 | 28.28 |