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. 2024 May 31;4(5):1380–1397. doi: 10.1158/2767-9764.CRC-24-0205

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© 2024 The Authors; Published by the American Association for Cancer Research

This open access article is distributed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license.

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LYVE-1 expression correlates with HA internalization. J774 macrophages untreated or treated with MCSF, IL4, dexamethasone, and EO771 conditioned media assessed for LYVE-1 expression (A) and CD44 expression (B) by flow cytometry. J774 macrophages untreated or treated with MCSF, IL4, dexamethasone, and EO771 conditioned media and pretreated with an isotype control or LYVE-1 blocking antibody followed by incubation with LMW-HA (C) or HMW-HA (D) and assessed by flow cytometry. Untreated and treated J774 macrophages assayed for hyaluronidase protein by ELISA (E) and hyaluronidase activity assay (F). G, Tumor lysates from Csf1r^fl/fl and Lyve^Cre Csf1r^fl/fl mice assayed by HA Activity ELISA (n = 5). H, EO771 tumors from Csf1r^fl/fl (gray) and Lyve^Cre Csf1r^fl/fl (blue) mice assessed for HA by ELISA and normalized by weight. J774 macrophages untreated or treated and incubated with LMW-HA (I) or HMW-HA (J) and assessed for HA internalization by flow cytometry. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Each dot represents one replicate.