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SDS–15% polyacrylamide gel of the fractions obtained during purification of Cox. Approximately 2 μg of protein was loaded. Lane 1, marker proteins (sizes in kilodaltons); lane 2, crude cell extract; lane 3, 25% ammonium sulfate precipitate; lane 4, Sephacryl S-200 Cox-containing fraction; lane 5, concentrated S-200 fraction.
