TABLE 2.
Bacterial strains and plasmids
| Bacterial strain or plasmid | Pertinent feature(s)a | Origin or reference |
|---|---|---|
| Strains | ||
| AG1688 | E. coli MC1061 F′128 lacIqlacZ::Tn5 | 13 |
| AG1688 (λ112OSPS) | AG1688 containing λ112 OSPS with lacZ driven by λ OSPS and a strong upstream lambda operator | 35 |
| AG1688 (λXZ970) | AG1688 containing λ112 OSPS with lacZ driven by λ OSPS and a 434 operator | 35 |
| BL21(DE3) | ompT hsdR hsdM lon E. coli B strain, T7 RNA polymerase under control of lacUV5 promoter | 28 |
| BL21(DE3) pLysE | Strain BL21(DE3) containing a pACYC184 derivative expressing T7 lysozyme | 28 |
| C-1a | F− prototrophic E. coli C strain | 24 |
| C-1757 | Polyauxotrophic E. coli C strain str supD | 29 |
| C-6005 | P2 cox3 lysogenic C-1a | 21 |
| JH372 | AG1688 containing λ202 with lacZ driven by λ PROR | 13 |
| Plasmids | ||
| pEE720 | P2 cox gene cloned in pET-8c under control of the T7 promoter φ10 | This paper |
| pEE721 | pET8-c derivative containing cox2 gene (M42T); primers cox2-R and cox2-L were used | This paper |
| pEE722 | pET8-c derivative containing cox3 gene (G22E); primers cox3-R and cox3-L were used | This paper |
| pEE723 | pET8-c derivative containing cox4 gene (E54K); primers cox4-R2 and cox4-L2 were used | This paper |
| pEE724 | pET8-c derivative containing cox107 gene (R29H); primers cox107-R and cox107-L were used | This paper |
| pEE725 | pET8-c derivative containing cox129 gene (A69T); primers cox 129-R and cox129-L were used | This paper |
| pEE726 | pET8-c derivative containing cox130 gene (E16K); primers cox130-R and cox130-L were used | This paper |
| pEE727 | P2 cox gene cloned in frame downstream of λ cI indl in pJH391 | This paper |
| pEE728 | pJH391 derivative containing cox2 gene (M42T); primers cox2-R and cox2-L were used | This paper |
| pEE729 | pJH391 derivative containing cox3 gene (G22E); primers cox3-R and cox3-L were used | This paper |
| pEE730 | pJH391 derivative containing cox4 gene (E54K); primers cox4-R2 and cox4-L2 were used | This paper |
| pEE731 | pJH391 derivative containing cox107 gene (R29H); primers cox107-R and cox107-L were used | This paper |
| pEE732 | pJH391 derivative containing cox129 gene (A69T); primers cox129-R and cox129-L were used | This paper |
| pEE733 | pJH391 derivative containing cox130 gene (E16K); primers cox130-R and cox130-L were used | This paper |
| pEE734 | pJH391 derivative containing truncated cox gene, cox38c (amino acids 1–53); primers cox4-R and cox4-L were used | This paper |
| pEE735 | pACYC177 derivative containing wt cox gene | This paper |
| pEE736 | pACYC177 derivative containing cox2 gene (M42T) | This paper |
| pEE737 | pACYC177 derivative containing cox4 gene (E54K) | This paper |
| pEE738 | pACYC177 derivative containing cox107 gene (R29H) | This paper |
| pEE739 | pACYC177 derivative containing cox129 gene (A69T) | This paper |
| pEE740 | pACYC177 derivative containing cox130 gene (E16K) | This paper |
| pEE741 | pKK232-8 derivative containing the cat gene under control of the P4 P11 promoter | Xiushan Wu, unpublished data |
| pET-8c | pBR322 derivative containing T7 promoter φ10 | 28 |
| pFG157 | λ cI indl driven by lacUV5 promoter | 13 |
| pJH370 | Fusion between λ cI indl and the leucine zipper of GCN4 driven by the lacUV5 promoter | 13 |
| pJH391 | First 132 nt of λ cI indl fused with lacZ driven by lacUV5 promoter | 13 |
| pJH622 | Fusion between λ cI indl and the reengineered leucine zipper of GCN4 driven by the lacUV5 promoter | 35 |
| pKH101 | N-terminal DNA-binding domain of λ cI indl | 13 |
| pSS27-5 | Ampicillin-sensitive derivative of pACYC177 | 21 |
| pSS39-6 | pKK232-8 derivative containing the cat gene under the control of the P2 Pc promoter, and the early Pe promoter is inactivated | 21 |
| pZ150 | pBR322 derivative with an M13 single-strand origin | 34 |
a
wt, wild type; nt, nucleotide(s)