TABLE 1.
Bacterial strains used
| Strain | Genotype | Reference(s) or source |
|---|---|---|
| dnaE74 (formerly called polC74) | dnaE74(Ts) (G134R) | R. Moses (3, 37) |
| BS40 | metB metD Δ(proAB lac) rpsL (Smr) | 40 |
| BS40metD+ | metB Δ(proAB lac) rpsL (Smr) | From BS40 by transduction |
| CC107 | ara Δ(lac proB) XIII/F′ lacIZ proB+ | 5 |
| CSH143 | ara Δ(gpt-lac)5 gyrA/F′ lac proA+B+ | 24 |
| MutS | lacZ53(Am) λ−mutS215::Tn10 (Tetr) thyA36 IN(rrnD-rrnE)1 rha-5 metB1 deoC2 | E. coli Genetic Stock Center (39) |
| FC755 | araD Δ(lac-proB)XIIIthi/F′ Δ (lacIZ) pro+ | R. Schaaper and P. Foster |
| DB1319 | λ−tyrT58(AS)trp-89::Tn5 recA938 (Camr)::Tn9-200 recD104(Nuc−) hsdR2 mdoB202::Tn10 | E. coli Genetic Stock Center (52) |
| S90C | ara Δ(lac proB)XIIIrpsL(Smr) | J. Miller |
| BS40dnaE74 | metB Δ(proAB lac) dnaE74(Ts) rpsL | This study |
| BS40dnaE173 | metB Δ(proAB lac) dnaE173 (E612K) rpsL | 22, 26 |
| BS40dnaE1336 | metB Δ(proAB lac) dnaE1336 (F347I) rpsL | |
| BS40dnaE1337 | metB Δ(proAB lac) dnaE1337 (D630G) rpsL | |
| BS40dnaE1338 | metB Δ(proAB lac) dnaE1338 (K655R) rpsL | |
| BS40dnaEn/F′CC107 | metB Δ(proAB lac) dnaEn rpsL/F′ lacIZ proB+ | |
| BS40dnaEn/F′CSH143 | metB Δ(proAB lac) dnaEn rpsL/F′ lac proA+B+ | |
| BS40mutSdnaEn/F′CC107 | metB Δ(proAB lac) mutS215::Tn10 dnaEn rpsL/F′ lacIZ proB+ | This study, by transduction |
| BS40mutSdnaEn/F′CSH143 | metB Δ(proAB lac) mutS215::Tn10 dnaEn rpsL/F′ lac proA+B+ | This study, by transduction |
| BS40dnaE74(Ts) ΔrecA::cam/F′CC107 | metB Δ(proAB lac) dnaE74(Ts) rpsL recA::cam/F′ lacIZ proB+ | This study, by transduction from DB1319 |
| YG7207 (PF2145) | AB1157 ΔdinB::kan | 20 |
| FC1237 | ara Δ(lac proB) XIII ΔdinB::kan | P. Foster |
| PFI173 CC107ΔdinB | ara Δ(lac proB) XIII Δ dinB::kan/F′ lacIZ proB+ ΔdinB::kan | P. Foster |
| BS40ΔdinB/F′CC107ΔdinB | metB Δ(proAB lac) rpsL ΔdinB::kan/F′ lacIZ proB+ ΔdinB::kan | This study |
| BS40ΔdinB/F′CSH143ΔdinB | metB Δ(proAB lac) dnaE630 rpsL ΔdinB::kan/F′ lac proA+B+ ΔdinB::kan | |
| BS40mutSΔdinB/F′CC107ΔdinB | metB Δ(proAB lac) rpsL ΔdinB::kan/F′ lacIZ proB+ ΔdinB::kan | |
| BS40mutSΔdinB/F′CSH143ΔdinB | metB Δ(proAB lac) dnaE630 rpsL ΔdinB::kan/F′ lac proA+B+ ΔdinB::kan | |
| BS40dnaE1336ΔdinB/F′CC107ΔdinB | metB Δ(proAB lac) dnaE1336 rpsL ΔdinB::kan/F′ lacIZ proB+ ΔdinB::kan | |
| BS40dnaE1336ΔdinB/F′CSH143ΔdinB | metB Δ(proAB lac) dnaE1336 rpsL ΔdinB::kan/F′ lac proA+B+ ΔdinB::kan | |
| BS40mutSdnaE347ΔdinB/F′CC107ΔdinB | metB Δ(proAB lac) dnaE1336 rpsL ΔdinB::kan/F′ lac proA+B+ ΔdinB::kan | This study |
| BS40mutSdnaE347ΔdinB/F′CSH143ΔdinB | metB Δ(proAB lac) dnaE1336 rpsL ΔdinB::kan/F′ lac proA+B+ ΔdinB::kan |