TABLE 2.
Classification of phenotypes
| Strain | Relevant genotype | Relevant phenotypea |
|---|---|---|
| HB101(pDB126) | (trbB-trbM)+ (traF-traM)+oriT+ | Transfer + |
| HB101(pDB126ΔtrbC) | (trbB trbD-trbM)+ (traF-traM)+oriT+, trbC0 | Transfer − |
| HB101(pDB126trbCX00Z) | (trbB trbC+, mutation indicated, trbD-trbM)+ (traF-traM)+oriT+ | Transfer +/− |
| HB101(pWP471/pDB129) | traF+/(trbB-trbM)+ (traG-traM)+oriT+ traF0 | Transfer + |
| HB101(pDB129) | (trbB-trbM)+ (traG-traM)+oriT+ traF0 | Transfer − |
| HB101(pWP471X00Z/pDB129) | traF+, mutation indicated/(trbB-trbM)+ (traG-traM)+oriT+ traF0 | Transfer +/− |
| JE2571(pWP471/pML123) | traF+/(trbB-trbM)+ | Dps/pilus formation + |
| JE2571(pWP471/pML123ΔtrbC) | traF+/(trbB trbD-trbM)+trbC0 | Dps/pilus formation − |
| JE2571(pWP471/pML123trbCX00Z) | traF+/(trbB trbC+, mutation indicated, trbD-trbM)+ | Dps/pilus formation +/− |
| JE2571(pWP471/pML123) | traF+/(trbB-trbM)+ | Dps/pilus formation + |
| JE2571(pML123) | (trbB-trbM)+ | Dps/pilus formation − |
| JE2571(pWP471X00Z/pML123) | traF+, mutation indicated/(trbB-trbM)+ | Dps/pilus formation +/− |
| SCS1(pJH472/pRE178) | traF+/trbC+ | Pilin detection + |
| SCS1(pJH472) | traF+ | Pilin detection − |
| SCS1(pJH472/pRE178X00Z) | traF+/trbC+, mutation indicated | Pilin detection +/− |
| SCS1(pJH472X00Z/pRE178) | traF+, mutation indicated/trbC+ | Pilin detection +/− |
a
Transfer, DNA transfer abilities determined by conjugation experiments as described in Materials and Methods. Transfer +, transfer positive; transfer −, transfer negative. Donor-specific phage propagation (Dps) and pilus formation are characterized as positive (+) for Dps and pilus formation or negative (−) for Dps and pilus formation. Detection of TrbC precursors or pilin by Western blotting and MALDI-TOF-MS is indicated as positive (+, detection) or negative (−, no detection).