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. 2024 Mar 21;9(9):e176962. doi: 10.1172/jci.insight.176962

Figure 2. Effect of TTFields on tumor cell growth in 3D bone graft tumor model, with or without titanium screws in the treatment field.

Figure 2

KRIB-mLuc cells (A–C) and A549-mLuc cells (D–F) were 3D cultured in demineralized bone matrix scaffolds and exposed to 150 kHz TTFields for 14 days. Tumor cell growth was monitored by BLI on days 0 and 14 (A and D). Control groups had significantly higher BLI signal on day 14 relative to baseline (day 0) compared with TTFields-exposed cultures (B and E). Control groups also contained significantly more viable cells (CellTiter-Glo [CTG] assay) on day 14 than the TTFields-exposed groups (C and F). A549-mLuc cells were cultured in demineralized bone matrix scaffolds with or without titanium screws to model the pedicle screws implanted in patients (G) and exposed to 150 kHz TTFields or control for 14 days. Tumor cell growth was monitored by BLI on days 0 and 14 (H). Control groups had significantly higher BLI signal on day 14 than TTFields-exposed groups, relative to respective baseline values. Additionally, the close proximity of titanium screws had no effect on the antiproliferative effects of TTFields exposure (I). Values represent mean ± SEM from independent experiments (n = 3). *P < 0.05, ***P < 0.0005 by unpaired, 2-tailed t test.