Skip to main content
[Preprint]. 2024 May 21:2024.05.21.594597. [Version 1] doi: 10.1101/2024.05.21.594597

Table 1. Primer sequences used in this study.

Sequences are shown for all primers used in this study. Primer pairs labeled RNA were used for RT-qPCR to quantify viral transcripts. Primers labeled DNA were used for qPCR to quantify viral genome copy numbers.

RNA Primer Pair Sequences
HCMV UL17 FWD: 5’-GGC CGT CCT TCT ATT TTG GC-3’
REV: 5’-TCT CGG TGA CTA CAG CCT AG-3’
HCMV UL44 FWD: 5’-GGC GTG AAA AAC ATG CGT ATC AAC-3’
REV: 5’-TAC AAC AGC GTG TCG TGC TCC G-3’
HCMV UL86 FWD: 5’-CCC GAG CGT TAC AAT ATT CAC-3’
REV: 5’-AAC CCA TTC CAG GCG ATT-3’
HCMV UL122 FWD: 5’-CAG AAC TCG GTG ACA TCC T-3’
REV: 5’-CCG GTG CTA CTG GAA TCG-3’
HCMV UL123 FWD: 5’-TGA CCG AGG ATT GCA ACG A-3’
REV: 5’-CCT TGA TTC TAT GCC GCA CC-3’
HCMV UL135 FWD: 5’-GCG GTG TAC GTC GCT CTA C-3’
REV: 5’-GGA AAC TCG GGT TTA TCT ATC G-3’
HCMV UL138 FWD: 5’-TGA GAT CTT GGT CCG TTG G-3’
REV: 5’-GTC TGT TAT CCG CGA CGA C-3’
HCMV US27 FWD: 5’-TAA CAT TTG CGG CTA CCT G-3’
REV: 5’-GCA CCA TAC GGT TGT ACG TG-3’
Hexose-6-phosphate dehydrogenase (H6PD) FWD: 5’-GGA CCA TTA CTT AGG CAA GCA-3’
REV: 5’-CAG GGT CTC TTT CAT GAT GAT CT-3’
DNA Primer Pair Sequences
HCMV IncRNA β2.7kb FWD: 5’-TGT TCT TCT GGT TCA TTT CCT ATG-3’
REV: 5’-CGT GTC CGG TCC TGA TTC-3’
Ribonuclease P (RNAseP) FWD: 5’-GAC GGA CTG CGC AGG TTA-3’
REV: 5’-CCA TGC TGA AGT CCC ATG A-3’
HCMV UL141 FWD: 5’-GAT GTG GGC CGA GAA TTA TGA-3’
REV: 5’-ATG GGC CAG GAG TGT GTC A-3’
HCMV UL141 probe    5’-CGA GGG AGA GCA AGT T-3’