Fig 8. Dxr2-017 inhibits melanoma cell growth through induction of anoikis.

(A) Inhibition of melanoma cell growth. Cells were treated with different concentrations of Dxr2-017 and formation of colonies at eight days depicted. Data are expressed as percent of untreated control and are the mean ± SEM (N=3 replicates) of a single experiment. (B) No effect on cycle progression. M14 and SK-MEL-5 cells were treated for 8 days with 20 and 50 nM Dxr2-017, respectively, and phase of cell cycle determined by flow cytometry. Data are the mean ± SEM (N=3). *denotes P-value ≤0.05. (C) Transition to floating cells. Cells were treated for three days with different Dxr2-017 concentrations. Depicted are representative light photomicrographs at 20X; scale bar is150 μm. Blue and green arrows denote adherent and floating cells, respectively. (D) Cleaved caspase 3 is induced in floating cells. Cells were treated for 3 days, cell lysate from only adherent cells, or from adherent and floating cells combined, was probed by Western blot for cleaved caspase 3. All experiments were repeated at separate times, yielding similar results.