TABLE 3.
Effects of CRP deletion at rhaBAD
| Promoter fusion | β-Galactosidase sp acta
|
|
|---|---|---|
| crp+ | Δcrp | |
| Φ(rhaB-lacZ)Δ226 | 414 | 0.13 |
| Φ(rhaB-lacZ)Δ110 | 408 | 0.09 |
| Φ(rhaB-lacZ)Δ84 | 9.1 | 0.18 |
a
β-Galactosidase specific activity was measured from single-copy rhaB-lacZ fusions in a wild-type crp or crp deletion strain. Cultures were grown in MOPS media containing fructose, l-rhamnose, and 2 mM cAMP. Standard errors were less than 17% of the average units except for that for Φ(rhaB-lacZ)Δ110 Δcrp, which was 35%, and that for Φ(rhaB-lacZ)Δ226Δcrp, which was 43%.