TABLE 7.
Effect of overexpressing α-Δ235 at rhaSR
| Promoter fusion | β-Galactosidase sp acta
|
|||
|---|---|---|---|---|
|
rhaSR+
|
ΔrhaSR
|
|||
| Wt α-CTD | α-Δ235 | Wt α-CTD | α-Δ235 | |
| Φ(rhaS-lacZ)Δ216 | 89 | 47 | 0.75 | 0.29 |
| Φ(rhaS-lacZ)Δ128 | 109 | 38 | 6.1 | 0.46 |
| Φ(rhaS-lacZ)Δ90 | 1.3 | 0.1 | 0.018 | 0.023 |
a
β-Galactosidase specific activity was measured from single-copy rhaS-lacZ fusions in either wild-type rhaSR or rhaSR deletion strain backgrounds transformed with plasmids encoding either wild-type α-CTD or α-Δ235. Cultures were grown in MOPS media containing glycerol, l-rhamnose, and ampicillin. Standard errors were less than 33% of the average units. Wt, wild type.