MATERIALS
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals | ||
| Octopamine (OA) | Tocris | Cat #2242 |
| Tyramine (TA) | Sigma-Aldrich | Cat #V900670 |
| Dopamine (DA) | Sigma-Aldrich | Cat #H8502 |
| Adenosine (Ado) | Tocris | Cat#3624 |
| All-trans-retinal | Sigma-Aldrich | Cat #R2500 |
| Acetylcholine (ACh) | Solarbio | Cat #G8320 |
| 5-hydroxytryptamine (5-HT) | Tocris | Cat #3547 |
| γ-aminobutyric acid (GABA) | Tocris | Cat #0344 |
| Mecamylamine (Meca) | Sigma-Aldrich | Cat #M9020 |
| Drosophila sNPF1 | GL Biochem (Shanghai) Ltd | AQRSPSLRLRFa |
| Drosophila sNPF2 | GL Biochem (Shanghai) Ltd | WFGDVNQKPIRSPSLRL RFa |
| Drosophila sNPF3 | GL Biochem (Shanghai) Ltd | KPQRLRWa |
| Drosophila sNPF4 | GL Biochem (Shanghai) Ltd | KPMRLRWa |
| Culex sNPF1 | GL Biochem (Shanghai) Ltd | KAVRSPSLRLRFa |
| Culex sNPF2 | GL Biochem (Shanghai) Ltd | APQLRLRFa |
| Culex sNPF3 | GL Biochem (Shanghai) Ltd | APSQRLRWa |
| hNPY | GL Biochem (Shanghai) Ltd | YPSKPDNPGEDAPAED MARYYSALRHYINLITRQRYa |
| CCha1 | GL Biochem (Shanghai) Ltd | SCLEYGHSCWGAHa |
| GL Biochem (Shanghai) Ltd | NSELINSLLSLPKNMNDAa | |
| FMRFa | GL Biochem (Shanghai) Ltd | SVKQNDFMHFa |
| AstA | GL Biochem (Shanghai) Ltd | VERYAFGLa |
| Dh31 | GL Biochem (Shanghai) Ltd | TVDFGLARGYSGTQEA KHRMGLAAANFAGGPa |
| Cell lines | ||
| HEK293T | ATCC | Cat#CRL-3216;RRID:CVCL_0063 |
| HTLAcellsforTangoassay | GiftfromBryanL.Roth | N/A |
| Fly strains | ||
| UAS-sNPF1.0 (chr2) | This study | N/A |
| UAS-sNPF1.0 (chr3) | This study | N/A |
| R13F02-Gal4 | Yi Rao, Peking University | BDSC: 48571 |
| MB247-LexA | Yi Zhong, Tsinghua University | N/A |
| UAS-Syt1-sgRNA (chr2) | This study | N/A |
| UAS-Syt4-sgRNA (chr2) | This study | N/A |
| UAS-Syt7-sgRNA (chr2) | This study | N/A |
| UAS-Sytα-sgRNA (chr2) | This study | N/A |
| UAS-Sytβ-sgRNA (chr2) | This study | N/A |
| UAS-ACh3.0 (chr3) | Jing et al.26 | BDSC: 86550 |
| LexAop-ACh3.0 (chr2) | Jing et al.26 | BDSC: 86551 |
| sNPF-atttp | Yi Rao, Peking University | BDSC:84574 |
| UAS-ca9.M6 | Yi Rao, Peking University | N/A |
| UAS-CsChrimson-mCherry / CyO; TM2 / TM6B | Chuan Zhou, Institute of Zoology, CAS | BDSC: 82181 |
| nSyb-gal4 / TM2 | Zhangwu Zhao, China Agricultural University | N/A |
| UAS-TeTxLC.tnt | Bloomington Drosophila Stock Center | BDSC: 28837 |
| Recombinant constructs | ||
| pDisplay vector | Invitrogen | Cat#V66020 |
| pDisplay-sNPF1.0-IRES-mCherry-CAAX | This study | N/A |
| pDisplay-sNPFmut-IRES-mCherry-CAAX | This study | N/A |
| UAS-sNPF1.0 | This study | N/A |
| UAS-sNPFmut | This study | N/A |
| sNPF1.0-SmBit | This study | N/A |
| Culex-sNPFR-SmBit | This study | N/A |
| pTango-sNPF1.0 | This study | N/A |
| pTango-culex-sNPFR | This study | N/A |
| Software and algorithms | ||
| OriginPro | OriginLab | 2022 |
| ImageJ | NIH | https://imagej.nih.gov/ij/; RRID: SCR_003070 |
| Arduino Uno | Arduino.cc | https://www.arduino.cc/en/Guide/ArduinoUno; RRID:SCR_017284 |