Figure 2.

Analysis of the cysteine acetylome following subcellular fractionation of mouse liver into cytoplasmic, mitochondrial, and nuclear fractions.

A. The summed intensity of cysteine acetylated peptides normalized to total peptide signal (in gray), and the number of unique S-acetylated peptides identified (in blue) for each subcellular compartment.

B. Heatmap showing distribution of cysteine acetylome in each subcellular compartment.

C. PCA of the cysteine acetylome in cytoplasmic, mitochondrial, and nuclear subcellular compartments. Scree plot showing the relative contribution of each of the first 10 components.

D. Ven diagram showing extent of overlap of the cysteine acetylome between subcellular compartments as measured by shared sites of cysteine acetylation. E. Pathway overrepresentation analysis of the cysteine acetylome for each compartment. Showing pathways with p-value < 0.01, adj. p-value < 0.05. Pathway overrepresentation analysis was performed using cysteine acetylated genes for each compartment against the list of all genes identified in that compartment as background.