Fig 4: Effect of blocking MAPK and NF-kB signaling in viral RNA mimic induced inflammatory and antiviral cytokine response.

Murine BMDMs were treated with each specific inhibitor of NF-kB and MAPK pathways for 2 hrs followed by stimulation with R848 and or Poly I:C each with 10 ug/ml. Western blot showing the inhibitory effect of Trametinib, Losmapimod, BAY, and JNKi on p-ERK, p-P38, p-P65, and p-JNK respectively (A) Protein levels of cytokines and interferons (B and C) and mRNA levels of ISGs (D and E) were assessed at 24 hours post-stimulation. Data are representative of 2-3 independent experiments (B-E). Statistical significance was determined using Student’s t-test with *P < .05 , **P < .01, **P < .001 and ****P < .0001.