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[Preprint]. 2024 May 25:2024.05.25.595513. [Version 1] doi: 10.1101/2024.05.25.595513

Table 3. Thermal stabilisation of full-length CNPase upon NbCNP binding.

Tm values were determined using DSF (N=3). Nanobody 8D was additionally studied using label-free nanoDSF.

sample Tm (°C) ΔTm (°C)
CNPase
DSF 59.2 ±0.1 -
nanoDSF 59.7 ±0.03 -
+7E 61.3 ±0.3 +2.1
+5E 76.2 ±0.3 +17.0
+8C 68.4 ±0.5 +9.2
+8D
DSF 77.2 ±0.3 +18.0
nanoDSF 77.4 ±0.1 +17.7
+10E 63.4 ±0.6 +4.2