Table 3. Thermal stabilisation of full-length CNPase upon NbCNP binding.

T_m values were determined using DSF (N=3). Nanobody 8D was additionally studied using label-free nanoDSF.

sample	Tm (°C)	ΔTm (°C)
CNPase
DSF	59.2 ±0.1	-
nanoDSF	59.7 ±0.03	-
+7E	61.3 ±0.3	+2.1
+5E	76.2 ±0.3	+17.0
+8C	68.4 ±0.5	+9.2
+8D
DSF	77.2 ±0.3	+18.0
nanoDSF	77.4 ±0.1	+17.7
+10E	63.4 ±0.6	+4.2