Table 3. Thermal stabilisation of full-length CNPase upon NbCNP binding.
Tm values were determined using DSF (N=3). Nanobody 8D was additionally studied using label-free nanoDSF.
sample | Tm (°C) | ΔTm (°C) |
---|---|---|
CNPase | ||
DSF | 59.2 ±0.1 | - |
nanoDSF | 59.7 ±0.03 | - |
+7E | 61.3 ±0.3 | +2.1 |
+5E | 76.2 ±0.3 | +17.0 |
+8C | 68.4 ±0.5 | +9.2 |
+8D | ||
DSF | 77.2 ±0.3 | +18.0 |
nanoDSF | 77.4 ±0.1 | +17.7 |
+10E | 63.4 ±0.6 | +4.2 |