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A. Sequence alignment of the five NbCNPs. Cys residues are indicated in yellow. B. DSF analysis of Nb stability. C. Pulldown assay indicates all Nbs bind to the CNPase catalytic domain. FL-CNP, full-length CNPase; CNPcat, CNPase catalytic domain; CNPcat(ext), CNPase catalytic domain with C-terminal tail. Pre, sample loaded onto affinity matrix; UB, unbound fraction; W1–3, wash fractions; Elu, eluted fraction.
