Figure 5. CNPase catalytic activity in the presence of selected nanobodies.
A. Initial rates of 2′,3′-cNADP+ hydrolysis. B. Lineweaver-Burk plot indicates non-competitive inhibition by nanobody 10E. C. Non-competitive Dixon plot in the presence of nanobody 10E. [I] denotes the concentration of 10E, and the x-axis intercept gives apparent −2Ki.