Table 1. Crystallisation and structure solution of CNPase-nanobody complexes.

MIB; buffer mixture of malonic acid, imidazole, and boric acid.

Nanobody	5E	7E	8C	8D	10E
Temperature (°C)	20	8	8	8	20
Well solution	0.2 M Na/K phosphate pH 7.0, 25% PEG3350	0.1 M MIB pH 4.0, 25% PEG3350	0.1 M MIB pH 8.0, 25% PEG1500	0.1 M phosphate-citrate pH 4.2, 18% PEG8000, 0.35 M NaCl	0.2 M Na malonate, 20% PEG3350
Protein concentration (mg/ml)	8.9	7.5	7.4	8.5	11.2
Cryoprotectant	25% PEG400	-	25% PEG400	30% glycerol	25% PEG400
Nanobody model PDB entry	6GWN chain C [46]	6H7J chain C [47]	6QV2 chain E [48]	AlphaFold2 [49] model of NbCNP-8D	7KN7 chain B [50]
Copies of complex in ASU	1	4	1	2	4