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Analysis of BlyA and BlyB protein levels in MNNG-induced B. burgdorferi. Cell extracts were prepared from B. burgdorferi B31 and CA-11.2A that were treated with MNNG (+) or left untreated (−) as described in Materials and Methods. Five microliters of each fraction was analyzed for BlyA (A) or BlyB (B) protein content by SDS-PAGE and Western blotting.
