Extended Data Fig. 5.

LOAD risk allele of PICALM impairs MG phagocytosis of Aβ. (a) Representative fluorescence images of iMG showing time-dependent phagocytosis of Aβ-pHrodo (red). (b) Quantification of Aβ-pHrodo phagocytosis in iMG of CD04 and CD09 lines. Data are from 2–3 independent wells of 4 FOVs each. Two-side unpaired t-test was used; * P<0.05, ** P<0.01, ***, P<0.001; error bar, SEM. (c) Cytochalasin D (10 μM) nearly completely inhibits phagocytosis. Cell mask stains for the plasma membrane and nuc blue stains nucleus in living cells. Time shows min after adding myelin pHrodo. Scale bar, 100 μm.