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. 2024 May 31;19(5):e0294089. doi: 10.1371/journal.pone.0294089

Table 2. RNA quantity (ng/uL), RNA quality (RIN) and qPCR Cycle Threshold (CT) values for samples stored in RNAlater at -20°C (Group A), 4°C (Group B) and 19°C (Group C) for one week prior to RNA isolation.

Sample Number Horse Number Sample storage temperature RNA (ng/uL) RIN CT value CT value
H3F3A RPL19
1 1 -20°C 446.23 9.4 20.55 19.10
2 2 -20°C 608.88 9 22.61 19.41
3 3 -20°C 798.87 9.9 21.18 20.22
4 4 -20°C 211.31 9.6 21.42 20.53
5 1 4°C 432.85 10 20.67 19.71
6 2 4°C 150.42 9.8 20.31 19.94
7 3 4°C 430.10 9.7 20.68 19.17
8 4 4°C 81.78  9.4 20.85 19.59
9 1 19°C 248.82 9.5 21.57 19.77
10 2 19°C 448.19 9.7 20.52 20.38
11 3 19°C 979.98 8.2 20.99 19.49
12 4 19°C 226.17 9.6 20.26 19.61