Table 2. RNA quantity (ng/uL), RNA quality (RIN) and qPCR Cycle Threshold (CT) values for samples stored in RNAlater at -20°C (Group A), 4°C (Group B) and 19°C (Group C) for one week prior to RNA isolation.
Sample Number | Horse Number | Sample storage temperature | RNA (ng/uL) | RIN | CT value | CT value |
---|---|---|---|---|---|---|
H3F3A | RPL19 | |||||
1 | 1 | -20°C | 446.23 | 9.4 | 20.55 | 19.10 |
2 | 2 | -20°C | 608.88 | 9 | 22.61 | 19.41 |
3 | 3 | -20°C | 798.87 | 9.9 | 21.18 | 20.22 |
4 | 4 | -20°C | 211.31 | 9.6 | 21.42 | 20.53 |
5 | 1 | 4°C | 432.85 | 10 | 20.67 | 19.71 |
6 | 2 | 4°C | 150.42 | 9.8 | 20.31 | 19.94 |
7 | 3 | 4°C | 430.10 | 9.7 | 20.68 | 19.17 |
8 | 4 | 4°C | 81.78 | 9.4 | 20.85 | 19.59 |
9 | 1 | 19°C | 248.82 | 9.5 | 21.57 | 19.77 |
10 | 2 | 19°C | 448.19 | 9.7 | 20.52 | 20.38 |
11 | 3 | 19°C | 979.98 | 8.2 | 20.99 | 19.49 |
12 | 4 | 19°C | 226.17 | 9.6 | 20.26 | 19.61 |