FIG. 2.

SDS-PAGE gel showing the purification of Rv2152c (A) and MLCB268.01c (B). Whole-cell lysate of uninduced E. coli BL21(DE3) cells harboring pSM201 and pSM206 (lanes 1A and 1B, respectively), whole-cell lysates of isopropyl-β-d-thiogalactopyranoside-induced E. coli BL21(DE3) cells overproducing M. tuberculosis and M. leprae MurC (Lanes 2A and 2B, respectively), and clarified cell extracts (supernatant) obtained by centrifugation at 30,000 × g of the whole-cell lysate (lanes 3A and 3B). Note that most of the overexpressed protein was insoluble and hence gives the impression of lesser expression. Purified MurC proteins are shown in lanes 4A and 4B. In each panel, the positions of molecular size markers are shown on the left.