Fig. 1.

Quantitative analysis of Tr1 and CD4⁺IL-10⁺ lymphocytes in T2DM. Peripheral blood mononuclear cells from patients with T2DM and healthy controls were analyzed by flow cytometry for Tr1 cells (CD4⁺CD49⁺LAG-3⁺IL-10⁺) and CD4⁺IL-10⁺ lymphocytes, as stated in “Materials and Methods”. A Flow cytometry strategy for the analysis of CD4⁺CD49⁺LAG-3⁺IL-10⁺. Data of a representative healthy control are shown. B Fold increase of % positive/% control levels of Tr1 cells in the peripheral blood from healthy controls and patients with mild (and well-controlled) and severe disease. C Levels of Tr1 cells, expressing or not Foxp3 (black and grey bars, respectively) in the peripheral blood from healthy controls and patients with T2DM. D Proportion of CD4⁺IL-10⁺ lymphocytes in the peripheral blood from healthy controls and patients with mild and severe T2DM. Data correspond to the mean and SEM. *p < 0.05; ***p < 0.001