Table 6.
Cardiac tissue results
| Donor | Tissue classification | Tissue | Sample ID | Subset | Processing information | sgRNA | gRNA | Mean Ct-GAPDH |
|---|---|---|---|---|---|---|---|---|
| D1 | Cardiac | Ascending aorta | D1.07.A | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 25.419 |
| D1 | Cardiac | Ascending aorta | D1.07.B | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 24.132 |
| D1 | Cardiac | Ascending aorta | D1.07.C | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 26.067 |
| D1 | Cardiac | Pulmonic valve | D1.08.A | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 23.783 |
| D1 | Cardiac | Pulmonic valve | D1.08.B | Replicate Sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 23.797 |
| D1 | Cardiac | Pulmonic valve | D1.08.C | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 24.65 |
| D3 | Cardiac | Pulmonic valve | D3.01.A | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 19.782 |
| D3 | Cardiac | Pulmonic valve | D3.01.B | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 19.532 |
| D3 | Cardiac | Pulmonic valve | D3.01.C | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 18.766 |
| D3 | Cardiac | Pulmonary Artery (Segment) | D3.02.A | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 24.639 |
| D3 | Cardiac | Pulmonary Artery (Segment) | D3.02.B | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 24.663 |
| D3 | Cardiac | Pulmonary Artery (Segment) | D3.02.C | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 22.758 |
| D7 | Cardiac | Pulmonary Artery (Segment) | D7.01.A | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 23.99 |
| D7 | Cardiac | Pulmonary Artery (Segment) | D7.01.B | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 27.149 |
| D7 | Cardiac | Pulmonary Artery (Segment) | D7.01.C | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 26.764 |
| D8 | Cardiac | Hemi Pulmonary Artery (Right) | D8.01.A | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 17.41 |
| D8 | Cardiac | Hemi Pulmonary Artery (Right) | D8.01.B | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 21.218 |
| D8 | Cardiac | Hemi Pulmonary Artery (Right) | D8.01.C | Replicate sample | Dissection, Antibiotics, Cryopreserved | Negative | Negative | 20.404 |
| D9 | Cardiac | Aortic valve | D9.01.A | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 31.421 |
| D9 | Cardiac | Aortic valve | D9.01.B | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 31.328 |
| D9 | Cardiac | Aortic valve | D9.01.C | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 32.06 |
| D9 | Cardiac | Pulmonic valve | D9.02.A | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 34.954 |
| D9 | Cardiac | Pulmonic valve | D9.02.B | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 33.815 |
| D9 | Cardiac | Pulmonic valve | D9.02.C | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 33.59 |
| D10 | Cardiac | Aortic valve | D10.03.A | Single sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 26.197 |
| D10 | Cardiac | Pulmonic valve | D10.04.A | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 33.74 |
| D10 | Cardiac | Pulmonic valve | D10.04.B | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 33.276 |
| D10 | Cardiac | Pulmonic valve | D10.04.C | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 34.716 |
| D11 | Cardiac | Aortic valve | D11.04.A | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 26.331 |
| D11 | Cardiac | Aortic valve | D11.04.B | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 26.69 |
| D11 | Cardiac | Aortic valve | D11.04.C | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 26.97 |
| D12 | Cardiac | Pulmonic valve | D12.01.A | Single sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | UND |
| D12 | Cardiac | Aortic valve | D12.03.A | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 23.434 |
| D12 | Cardiac | Aortic valve | D12.03.B | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 23.519 |
| D12 | Cardiac | Aortic valve | D12.03.C | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 32.084 |
| D13 | Cardiac | Aortic valve | D13.01.A | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 25.848 |
| D13 | Cardiac | Aortic valve | D13.01.B | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 26.596 |
| D13 | Cardiac | Aortic valve | D13.01.C | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 26.523 |
| D13 | Cardiac | Pulmonic valve | D13.02.A | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 25.703 |
| D13 | Cardiac | Pulmonic valve | D13.02.B | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 26.293 |
| D13 | Cardiac | Pulmonic valve | D13.02.C | Replicate Sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 27.497 |
| D14 | Cardiac | Pulmonic valve | D14.01.A | Replicate Sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 32.882 |
| D14 | Cardiac | Pulmonic valve | D14.01.B | Replicate Sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 32.753 |
| D14 | Cardiac | Pulmonic valve | D14.01.C | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 32.25 |
| D14 | Cardiac | Aortic valve | D14.02.A | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 28.36 |
| D14 | Cardiac | Aortic valve | D14.02.B | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 28.772 |
| D14 | Cardiac | Aortic valve | D14.02.C | Replicate sample | Dissection, Antibiotics, Flash Frozen | Negative | Negative | 28.257 |
This table provides cardiac tissue results. All samples were tested in quadruplicate for both subgenomic RNA (sgRNA) and genomic nucleocapsid RNA (gRNA) of SARS-CoV-2, and in duplicate for the housekeeping gene Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which served as the positive control. UND indicates an undetected GAPDH result