Table 8.
Donor | Tissue classification | Tissue | Sample ID | Subset | Processing information | sgN | wtN | Mean Ct-GAPDH |
---|---|---|---|---|---|---|---|---|
D2 | Musculoskeletal | Femur (Left) | D2.01.A | Endosteum | No processing | Negative | Negative | 28.061 |
D2 | Musculoskeletal | Femur (Left) | D2.01.B | Periosteum | No processing | Negative | Negative | 28.223 |
D2 | Musculoskeletal | Femur (Left) | D2.01.C | Cancellous Bone | No processing | Negative | Negative | 24.171 |
D2 | Musculoskeletal | Gracilis Tendon (Left) | D2.02.A | Replicate sample | No processing | Negative | Negative | 29.544 |
D2 | Musculoskeletal | Gracilis Tendon (Left) | D2.02.B | Replicate sample | No processing | Negative | Negative | 29.19 |
D2 | Musculoskeletal | Gracilis Tendon (Left) | D2.02.C | Replicate sample | No processing | Negative | Negative | 29.51 |
D2 | Musculoskeletal | Tibia (Left) | D2.05.A | Endosteum | No Processing | Negative | Negative | 28.998 |
D2 | Musculoskeletal | Tibia (Left) | D2.05.B | Periosteum | No processing | Negative | Negative | 24.958 |
D2 | Musculoskeletal | Tibia (Left) | D2.05.C | Cancellous bone | No processing | Negative | Negative | 29.583 |
D4 | Musculoskeletal | Semitendinosus Tendon (Right) | D4.01.A | Replicate sample | No processing | Negative | Negative | 29.263 |
D4 | Musculoskeletal | Semitendinosus Tendon (Right) | D4.01.B | Replicate sample | No processing | Negative | Negative | 28.705 |
D4 | Musculoskeletal | Semitendinosus Tendon (Right) | D4.01.C | Replicate sample | No processing | Negative | Negative | 30.03 |
D4 | Musculoskeletal | Tibia (Left) | D4.02.A | Endosteum | No processing | Negative | Negative | 30.557 |
D4 | Musculoskeletal | Tibia (Left) | D4.02.B | Periosteum | No processing | Negative | Negative | 29.521 |
D4 | Musculoskeletal | Tibia (Left) | D4.02.C | Cancellous bone | No processing | Negative | Negative | 23.819 |
D4 | Musculoskeletal | Femur (Left) | D4.03.A | Endosteum | No processing | Negative | Negative | 26.66 |
D4 | Musculoskeletal | Femur (Left) | D4.03.B | Periosteum | No processing | Negative | Negative | 30.227 |
D4 | Musculoskeletal | Femur (Left) | D4.03.C | Cancellous bone | No processing | Negative | Negative | 23.393 |
D5 | Musculoskeletal | Semitendinosus Tendon (Right) | D5.01.A | Replicate sample | No processing | Negative | Negative | 30.722 |
D5 | Musculoskeletal | Semitendinosus Tendon (Right) | D5.01.B | Replicate sample | No processing | Negative | Negative | 28.714 |
D5 | Musculoskeletal | Semitendinosus Tendon (Right) | D5.01.C | Replicate sample | No processing | Negative | Negative | 29.516 |
D5 | Musculoskeletal | Tibia (Right) | D5.02.A | Endosteum | No processing | Negative | Negative | 22.375 |
D5 | Musculoskeletal | Tibia (Right) | D5.02.B | Periosteum | No processing | Negative | Negative | 21.736 |
D5 | Musculoskeletal | Tibia (Right) | D5.02.C | Cancellous bone | No processing | Negative | Negative | 22.038 |
D6 | Musculoskeletal | Achilles Tendon (Right) | D6.01.A | Replicate sample | No processing | Negative | Negative | 29.536 |
D6 | Musculoskeletal | Achilles Tendon (Right) | D6.01.B | Replicate sample | No processing | Negative | Negative | 29.911 |
D6 | Musculoskeletal | Achilles Tendon (Right) | D6.01.C | Replicate sample | No processing | Negative | Negative | 24.643 |
D6 | Musculoskeletal | Humerus (Right) | D6.02.A | Endosteum | No processing | Negative | Negative | 24.452 |
D6 | Musculoskeletal | Humerus (Right) | D6.02.B | Periosteum | No processing | Negative | Negative | 27.349 |
D6 | Musculoskeletal | Humerus (Right) | D6.02.C | Cancellous bone | No processing | Negative | Negative | 21.908 |
D15 | Musculoskeletal | Fascia Lata | D15.01.A | Replicate sample | Dissection, saline rinse | Negative | Negative | 27.013 |
D15 | Musculoskeletal | Fascia Lata | D15.01.B | Replicate sample | Dissection, Saline Rinse | Negative | Negative | 26.887 |
D15 | Musculoskeletal | Fascia Lata | D15.01.C | Replicate sample | Dissection, saline rinse | Negative | Negative | 29.475 |
D16 | Musculoskeletal | Fascia Lata | D16.01.A | Replicate sample | Dissection, saline rinse | Negative | Negative | 27.635 |
D16 | Musculoskeletal | Fascia Lata | D16.01.B | Replicate sample | Dissection, Saline Rinse | Negative | Negative | 29.245 |
D16 | Musculoskeletal | Fascia Lata | D16.01.C | Replicate sample | dissection, saline rinse | Negative | Negative | 28.883 |
D17 | Musculoskeletal | Fascia Lata | D17.01.A | Replicate sample | Dissection, saline rinse | Negative | Negative | 29.467 |
D17 | Musculoskeletal | Fascia Lata | D17.01.B | Replicate sample | Dissection, saline rinse | Negative | Negative | 27.725 |
D17 | Musculoskeletal | Fascia Lata | D17.01.C | Replicate sample | Dissection, saline rinse | Negative | Negative | 28.083 |
This table provides musculoskeletal tissue results. All samples were tested in quadruplicate for both subgenomic RNA (sgRNA) and genomic nucleocapsid RNA (gRNA) of SARS-CoV-2, and in duplicate for the housekeeping gene Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which served as the positive control