Fig. 12.

Flow cytometry of lymphocytes from the pig heart. a Sequential gating strategy for immunophenotyping of lymphocytes. Cells were fixed and permeabilized. All events were pre-gated on viable (Zombie Aqua⁻) singlets. DN (T): double-negative (T) cells, DP: double-positive cells. b Proportion of lymphocytes among total leukocytes from the different regions at the indicated points in time (d = days) post-MI or from sham hearts. c–d Proportions of lymphocyte subsets from c the infarct core or d the remote zone (n = 3–4 for each). e Foxp3 intracellular expression on CD4⁺ T cells from the infarct core (n = 3–4). Mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. pooled sham (two-way ANOVA with Dunnett’s multiple comparisons test)