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. 2024 Mar 15;119(3):453–479. doi: 10.1007/s00395-024-01036-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 13 — Flow cytometry of stem and myeloid progenitor cells from the porcine spleen. a Sequential gating strategy for the identification of stem cells (SC), myeloid progenitor cells (MPC) and myeloid cell subsets (M1, M2) among lymphoid lineage markers CD3 and CD21^a negative (lymph⁻) spleen (SPL) cells. Cells were fixed and permeabilized. All events were pre-gated on viable (Zombie Aqua⁻) singlets. b Proportions of spleen cell subsets among lymphoid lineage marker negative cells at the indicated points in time (d = days) post-MI and from sham animals (n = 3–4 each). c Proliferation rate of the individual spleen cell subsets as determined by intracellular Ki67 expression (PS: pooled sham). Mean ± SD; *p < 0.05, **p < 0.01 vs. pooled sham (two-way ANOVA with Dunnett’s multiple comparisons test)