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. 2024 May 31;14:12521. doi: 10.1038/s41598-024-63525-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Resveratrol induces mitophagy and mitochondrial biogenesis by regulating PGC1-α acetylation in MIN6 Scr pancreatic beta cells. (A) Western blotting analysis showing the study of different proteins from the different mitochondrial compartments for study mitophagy at 24 h in response to either rapamycin, CCCP or resveratrol. The graphs correspond to the quantification analysis of the protein changes in response to the different stimuli expressed in percentage (n = 3). (B) Immunoprecipitation analysis of PGC1-α for analyzing the acetylation status of the protein in response or not to resveratrol for 4 h in MIN6 Scr and Tsc2 shRNA cells. The graph corresponds to the quantification analysis showing the fold increase in the ratio of Ac-PGC1-α/PGC1-α (n = 3).