Fig. 1. Vaccination with irradiated iPSC-whole-cell lysate combined with RT leads to tumor remission.
a The identification and characterization of mouse iPSCs after 5 passages. Scale bar = 50 μm. b Schematic diagram of the irradiated iPSC vaccine and RT treatment. A total of 3 × 105 CT26 cells were subcutaneously inoculated into BALB/c mice for 6 days, and irradiated iPSCs or fibroblasts (1 × 105 cells, 50 Gy) were subcutaneously administered adjuvant GM-CSF (50 ng/shot) on Days 6, 11, 16 and 21. Local radiotherapy was given on Days 8, 13 and 18. The animal experiments were repeated twice, independently. c The representative image of the resected tumor tissues. Scale bar = 1 cm. d Tumor volume was calculated every 3 days (n = 8). e Tumor volume was calculated (Day 24, n = 8). f The resected tumor weight was measured (Day 24, n = 8). g The resected tumor tissues were analyzed by immunoblotting (n = 3). h The percentage of apoptotic cells (cleaved caspase-3+ cells) was evaluated by immunohistochemical analysis (n = 3). Scale bar = 30 μm. Significance was calculated with multiple t tests analysis and is presented as mean ± s.e.m. *p < 0.05; **p < 0.01 and ***p < 0.001.