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. 2024 May 31;10:267. doi: 10.1038/s41420-024-02039-7

Fig. 7. Analysis of serum cytokines and tissue effects in bleomycin and conodin A co-treated mice.

Fig. 7

A Monitoring and recording of body weight changes in control, BLM, and BLM+Conodin A groups from the day prior to cell injection till the day before euthanasia, using an electronic scale. B Temperature measurements of mice in control, BLM, and BLM+Conodin A groups, conducted with an electronic thermometer from the day before cell suspension injection to the day preceding euthanasia. C Serum from mice was isolated to evaluate IFN-γ levels using a specific detection kit. The concentration of IFN-γ was measured in three groups: control (Con), bleomycin only (BLM), and a combination of bleomycin with Conodin A (BLM + Conodin A). D Similarly, TNF-α levels in mouse serum were assessed using a dedicated detection kit. TNF-α concentrations were analyzed for the same three groups: Con, BLM, and BLM + Conodin A. E Tissue samples from the liver, spleen, lung, and tumor of mice in each group (Con, BLM, and BLM + Conodin A) underwent Hematoxylin and Eosin (H&E) staining. Microscopic examination of these stained tissues was performed to evaluate structural changes and damage, with results meticulously documented. Statistical significance across the groups is denoted as ***P < 0.001.