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. 2024 May 31;14:12559. doi: 10.1038/s41598-024-63337-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of expression of viral proteins among HEP, HEP-10V and HEP-30V in Vero cells. Vero cells were inoculated with each virus at an M.O.I. of 5 and harvested every day. (a) Rabies virus (RABV) glycoprotein (G protein) and nucleoprotein (N proteins) were visualized using polyclonal rabbit anti-serum against each protein. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a housekeeping protein, was detected by monoclonal mouse antibody for use as a loading control. (b) Virus-specific bands were quantified using ImageJ software (National Institutes of Health, Bethesda, MD, USA) and levels of RABV G and N proteins were normalized to those of GAPDH. The means and S.D. were calculated from two independent experiments. Significant differences are indicated (*: p < 0.05, **: p < 0.01, ***: p < 0.001) after application of two-way ANOVA followed by Tukey.