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. 2024 May 31;14:12559. doi: 10.1038/s41598-024-63337-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of virus-neutralizing antibody titers against HEP, HEP-10V and HEP-30V using vaccinated rabbit sera. Sera were collected from each of four rabbits (H1, H2, A1, A2). H1 and H2 were inoculated with the human-RABV vaccine (Rabipur, GSK Biologicals, Wavre, Belgium) and A1 and A2 were inoculated with the animal-RABV vaccine (KMB, KM Biologics, Kumamoto, Japan). Virus-neutralizing antibody (VNA) titers were determined by rapid fluorescent focus inhibition tests (RFFITs) and quantified using the World Health Organization (WHO) international units (IU/ml). Means and S.D. were calculated from four independent experiments. Significant difference is indicated (*: p < 0.05) in the neutralizing activities against HEP and HEP-30V in the H1 rabbit serum after application of two-way ANOVA followed by Tukey.