TABLE 2.
Subcellular localization of tartrate-resistant acid phosphatase and SapM activity in M. tuberculosis
| Subcellular fraction | Acid phosphatase activitya (nmol of pNP/min/mg of protein) | Isocitrate dehydrogenase activityb (pmol of NADPH/ min/mg of protein) |
|---|---|---|
| Culture filtrate | 800 ± 70 | 8.4 ± 2.6 |
| Cell wall | 190 ± 30 | NAc |
| Cell membrane | 170 ± 20 | NAc |
| Cytosol | 30 ± 4 | 88.0 ± 8.4 |
a
Activity was determined in a microtiter plate assay.
b
Isocitrate dehydrogenase activity was determined as described by Andersen et al. (1).
c
NA, no activity was detected.