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. Author manuscript; available in PMC: 2024 Jun 1.
Published in final edited form as: Protein Expr Purif. 2023 Jun 15;210:106322. doi: 10.1016/j.pep.2023.106322

Fig. 2. Initial 100 mL purification trials.

Fig. 2.

(A) Purification of full length FAM210A fusion protein from BL21(DE3) cells using a 0.25 mM IPTG induction for 3.5 hr at 37°C. Lane 1: Protein marker ladder. Lane 2: Uninduced cells. Lane 3: Induced cells. Lane 4: Cell debris pellet. Lane 5: Cell lysate flowthrough. Lane 6: Cell lysate supernatant. Lane 7: Cell lysate wash. Lane 8: Elution 1. Lane 9: Elution 2. Lane 10: Elution 3. Lane 11: Solubilized membrane flowthrough. Lane 12: Solubilized membrane wash. Lane 13: Membrane elution 1. Lane 14: Membrane elution 2. (B) Purification of dMTS FAM210A fusion protein using BL21(DE3) cells (100 mL) with 0.25 mM IPTG induction for 3.5 hr at 37°C. * is placed at the solubilized FAM210A fusion protein that was extracted from bacterial membranes. Lane 1: Protein marker ladder. Lane 2: Uninduced cells. Lane 3: Induced cells. Lane 4: Cell debris pellet. Lane 5: Cell lysate flowthrough. Lane 6: Cell lysate supernatant. Lane 7: Cell lysate wash. Lane 8: Elution 1. Lane 9: Elution 2. Lane 10: Elution 3. Lane 11: Solubilized membrane flowthrough. Lane 12: Solubilized membrane wash. Lane 13: Membrane Elution 1. Lane 14: Membrane elution 2.