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. Author manuscript; available in PMC: 2024 Jun 1.
Published in final edited form as: Protein Expr Purif. 2023 Jun 15;210:106322. doi: 10.1016/j.pep.2023.106322

Fig. 4. SDS-PAGE gel showing the results of each purification step.

Fig. 4.

Gel was stained with Coomassie G-250 to visualize proteins. Both the fusion protein, the cleaved FAM210A-dMTS (*), and a contaminant FAM210A-associated protein (#) are labelled on the image. Lane 1: first-step nickel affinity chromatography. Lane 2: TEV cleavage and second-step nickel affinity chromatography. Lane 3: Ion exchange chromatography.