TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant features | Source or reference |
|---|---|---|
| Strains | ||
| M. smegmatis | ||
| mc2155 | ept1 ddlA+ | 16 |
| SMEG35 | ept1 ddlA1(Ts) | This study |
| E. coli XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 SupE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene, La Jolla, Calif. |
| Plasmids | ||
| pBluescript SK− | E. coli cloning vector | Stratagene |
| pMD30 | E. coli-mycobacterial shuttle vector | 7 |
| pQE31 | Protein expression vector | Qiagen, Chatsworth, Calif. |
| pAEB222 | M. smegmatis-derived cosmid containing ddlA | This study |
| pAEB224 | 1.4-kb Sau3AI fragment containing ddlA cloned into pMD30 | This study |
| pAEB242 | Wild-type ddlA gene cloned into pQE31 | This study |
| pAEB243 | SMEG35 ddlA1 gene cloned into pQE31 | This study |