Fig. 6.

Representative images of the immunofluorescence staining (200 ×) of 4-HNE (a), NOX2 (b), and NOX4 (c) expression in conjunctiva dissected from the experimental dry eye model. The RCI group saw reduced expression levels of 4-HNE, NOX2, and NOX4, and the decrease was comparable to that in the PBS, PDE, and Lifitegrast groups (yellow arrows). Red, 4-HNE and NOX2; green, NOX4; blue, nuclear DAPI staining. Scale bar: 100 μm, n = 3 per group; PBS, phosphate buffered saline; PDE, 1% prednisolone acetate; Lifite, 5% Lifitegrast; RCI, 1.0% RCI001; 4-HNE, 4-hydroxy-2-nonenal; NOX2, nicotinamide adenine dinucleotide phosphate oxidase 2; NOX4, nicotinamide adenine dinucleotide phosphate oxidase 4; DAPI, 4′,6-diamidino-2-phenylindole