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. 2024 Jun 1;43:156. doi: 10.1186/s13046-024-03083-y

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© The Author(s) 2024, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — ILK inhibition blocks the OCSC phenotype and regulates Fzd7 expression. A CSC genes down-regulated in OVCAR-3 spheroids by cpd-22 (0.5 μM) treatment compared to DMSO control (≥ 6.0-fold change). B ALDH1A1, Sox-2, and Nanog mRNA levels measured by qRT-PCR in OVCAR-3 (left panel) and HEY-A8 (right panel) spheroids treated with DMSO control or cpd-22 (N = 3; ****P < 0.0001). C QRT-PCR for Fzd7 and c-Myc mRNA levels in OVCAR-3 (left panel) and HEY-A8 spheroids (right panel) treated with cpd-22 vs. DMSO (N = 3; **P < 0.01, ***P < 0.001, ****P < 0.0001). D WB for A-β-catenin, Fzd7, p-ILK^Ser246, ILK, and GAPDH in OVCAR-3 and HEY-A8 spheroids shCtr and shILK (clone #3) (left panel). Densitometry quantifies A-β-catenin and Fzd7 expression levels and p-ILK^Ser246/ILK ratio (N = 3; *P < 0.05, ***P < 0.001, ****P < 0.0001). E WB for A-β-catenin, Fzd7, p-ILK^Ser246, ILK, and GAPDH in UHB-2012–06 HGSOC primary spheroids treated with DMSO or cpd-22 (0.5 μM) (left panel). Densitometry quantifies A-β-catenin and Fzd7 expression levels and p-ILK^Ser246/ILK ratio (N = 3; *P < 0.05 and **P < 0.01). F WB for p-ILK^Ser246, ILK, Fzd7, and GAPDH in HEY-A8 shCtr and shFzd7 (clone #2) grown as spheroids and treated or not for 7 days with Wnt-3A (left panel). Densitometry quantifies Fzd7 expression levels and p-ILK^Ser246/ILK ratio (right panel) (N = 3; *P < 0.05, ***P < 0.001, ****P < 0.0001). G. WB for A-β-catenin, p-GSK-3α/β^Ser21/9, GSK-3α/β, Fzd7, and GAPDH in HEY-A8 shCtr and shFzd7 (clone #2) grown as spheroids and treated or not for 7 days with Wnt-3A (left panel). Densitometry quantifies A-β-catenin and Fzd7 expression levels and p-GSK-3α/β^Ser21/9/GSK-3α/β ratio (right panel) (N = 3; *P < 0.05, **P < 0.01, ****P < 0.0001)