Fig. 4.

Knockdown of circSEC24A slowed OA progression via sponging miR-107-5p in vitro. (A) The levels of circSEC24A and miR-107-5p were determined by qRT-PCR. (B) Cell viability was monitored by MTT assay. (C) Cell apoptosis was assessed by Annexin V/PI staining followed by flow cytometry. (D) The protein levels of cleaved caspase 3, Bax and Bcl-2 were determined by Western blot. (E) The mRNA levels of Col2a1, ACAN, MMP13 and ADAMTS5 were determined by qRT-PCR. (F) The protein levels of Col2a1, ACAN, MMP13 and ADAMTS5 were determined by Western blot. (G) The secreted TNF-α, IL-6 and IL-10 levels were measured by ELISA assays. ∗P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001.