Skip to main content
. 2000 Feb;74(3):1061–1068. doi: 10.1128/jvi.74.3.1061-1068.2000

FIG. 5.

FIG. 5

LMP-1 reduces the endogenous EBNA-1 mRNA transcribed from Qp in type I cells. (A) Reduction of endogenous EBNA-1 mRNA in cells transfected with an LMP-1 expression plasmid. RPA was performed with GAPDH and EBNA-1 probes with various RNAs. Lane 1, yeast RNA; lane 2, total RNA from DG75, an EBV-negative cell line; lanes 3 to 5, RNAs from Eli-BL cells transfected with pcDNA3, pcLMP1 (5 μg), and pcLMP1 (10 μg), respectively; lanes 6 and 7, RNAs from Akata cells transfected with pcDNA3 and pcLMP1, respectively. The relative EBNA-1 mRNA levels are shown. Data were analyzed by normalizing EBNA-1 mRNA levels to the GAPDH level with the use of a PhosphorImager. In LMP-1-transfected cells, the average EBNA-1 mRNA level (and standard deviation) relative to pcDNA3-transfected cells from four experiments was 0.52 ± 0.09. One representative experiment is shown. (B) Stimulation of IRF-7 by LMP-1 in Eli-BL cells. RPA was performed with GAPDH and IRF-7 probes with various RNAs. Lane 1, yeast RNA; lanes 2 to 4, RNAs from Eli-BL cells transfected with pcDNA3, pcLMP1 (5 μg), and pcLMP1 (10 μg), respectively; lanes 5 and 6, RNAs from Akata cells transfected with pcDNA3 and pcLMP1 respectively. One representative experiment is shown.