FIG. 4.

Maximum-likelihood tree of the nucleotide sequences of C2/V3 clones (324 bp) from uncultured PBMCs of the family members at different sampling points. The tree was estimated by using the DNAML program in PHYLIP, version 3.572. Nineteen available sequences of HIV-1 subtype E with various geographic origins (26) were added as the outgroup. Numbers with arrows represent lengths and confidence limits of branches in the likelihood analysis. Branches with asterisks are significantly positive (P < 0.01).