Fig. 6.

Effect of PRL on antioxidant enzymes activity in rat astrocytes. Rat cortical astrocytes were pre-incubated in the absence or presence of either 10 nM prolactin (PRL), 100 μM STAT3 inhibitor S3I-201 or both for 24 h, then 400 μM hydrogen peroxide (H₂O₂) or vehicle (Veh) was added and incubated for 3 h. a Superoxide dismutase (SOD), b glutathione peroxidase (GPX), and c catalase (CAT) activities were detected in samples of each treatment group containing equal amounts of protein using the corresponding commercial assay. Data in a, b and c are means ± SEM of three independent experiments (n = 3) carried out in duplicate. One-way ANOVA followed by Tukey’s test. *p < 0.05, **p < 0.001 versus indicated group; n.s., no significant difference